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Product Information
Description |
SF3B14 rabbit polyclonal antibody |
Protein full name |
Splicing factor 3B subunit 6 |
Synonyms |
Pre-mRNA branch site protein p14, SF3b 14 kDa subunit, Sf3b6, Sf3b14 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse SF3B14 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Nucleus |
Predicted MW. |
14 kDa |
Observed MW. |
14 kDa |
Uniprot ID |
Q9Y3B4, P59708 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Human, Mouse, Rat |
1:500-1:1000 |
testis, colon |
IHC/IF |
Human, Mouse, Rat |
1:500-1:2000 |
brain, colon cancer, heart, liver |
Background
This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.
Images
|
Western blot analysis of SF3B14 (GB111446) at dilution of 1: 500 |
|
Immunohistochemistry of paraffin embedded human brain using SF3B14 (GB111446) at dilution of 1: 1000 (200x lens) |
|
Immunohistochemistry of paraffin embedded human colon cancer using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens) |
|
Immunohistochemistry of paraffin embedded mouse liver using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens) |
|
Immunohistochemistry of paraffin embedded rat heart using SF3B14 (GB111446) at dilution of 1: 1000 (300x lens) |
|
Immunofluorescence of paraffin embedded mouse brain using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens) |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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