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Anti -Heme-regulated inhibitor Rabbit pAb
价格：
在线咨询
货号：
KT012032
产品规格：
100 μL
Applications：
WB/IHC/IF
Species：
HMR/MR/MR
购买数量：
产品详情
操作说明
注意事项
Product Information
|
Description |
Heme-regulated inhibitor rabbit polyclonal |
|
Protein full name |
Eukaryotic translation initiation factor 2-alpha kinase 1 |
|
Synonyms |
Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Eif2ak1, Heme-controlled repressor, HCR, Heme-regulated inhibitor, Hri |
|
Immunogen |
Recombinant protein corresponding to Mouse Heme-regulated inhibitor |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. |
70 kDa |
|
Observed MW. |
70 kDa |
|
Subcellular location |
Cytoplasm |
|
Uniprot ID |
Q9BQI3, Q9Z2R9, Q63185 |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
HEK293 |
|
IHC |
Mouse, Rat |
1: 800-1: 1600 |
bone, lung, inflammation lung |
|
IF |
Mouse, Rat |
1: 500-1: 1000 |
bone, lung, inflammation lung |
Background
The Eif2ak1 Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells, controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties.
Images
|
|
Western blot analysis of Eif2ak1 (GB111094) at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse bone using Eif2ak1 (GB111094) at dilution of 1: 1000 |
|
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Immunohistochemistry analysis of paraffin-embedded rat lung using Eif2ak1 (GB111094) at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse inflammation lung using Eif2ak1 (GB111094) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室