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Anti -Cdk2 Rabbit pAb

价格: 在线咨询
货号:
KT011889
产品规格:
100 μL
Applications:
WB | IHC
Species:
H,M,R | H,M,R
购买数量:
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产品详情 操作说明 注意事项

Product Information


Description

Cdk2 rabbit polyclonal

Protein full name

Cyclin-dependent kinase 2

Synonyms

Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2, CDC28, CDC2A,
Cdc2 related protein kinase, p33(CDK2), MPF

Immunogen

KLH conjugated Synthetic peptide

corresponding to Human Cdk2

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW.

34 kDa

Observed MW.

34 kDa

Uniprot ID

P24941, P97377, Q63699




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 400-1: 800

spleen, thymus,

lymph nodes

IHC

Human, Mouse, Rat

1: 200-1: 800

B-lymphoma, spleen, thymus




Background


The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B).




Images


Western blot analysis of Cdk2

at dilution of 1: 800

Lane 1: HeLa cell lysate

Lane 2: K562 cell lysate

Lane 3: MCF7 cell lysate

Lane 4: Mouse spleen tissue lysate

Lane 5: Mouse thymus tissue lysate

Lane 6: Mouse lymph nodes tissue lysate

Lane 7: Rat thymus tissue lysate

Lane 8: Rat lymph nodes tissue lysate

Immunohistochemistry analysis of paraffin-embedded human B-lymphoma using Cdk2 at dilution of 1: 400

Immunohistochemistry analysis of paraffin-embedded mouse spleen using

Cdk2  at dilution of 1: 400

Immunohistochemistry analysis of paraffin-embedded rat spleen using

Cdk2 at dilution of 1: 400

Immunohistochemistry analysis of paraffin-embedded mouse thymus using

Cdk2 at dilution of 1: 400

图片 6.png

Immunohistochemistry analysis of paraffin-embedded rat thymus using

Cdk2 at dilution of 1: 400




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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