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产品详情
操作说明
注意事项
Product Information
|
Description |
PCNA rabbit polyclonal antibody |
|
Protein full name |
Proliferating cell nuclear antigen |
|
Synonyms |
PCNA, ATLD2, proliferating cell nuclear antige |
|
Immunogen |
Recombinant protein corresponding to Mouse PCNA |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Nucleus |
|
Predicted MW. |
29 kDa |
|
Observed MW. |
36 kDa |
|
Uniprot ID |
Q12004, P17918, P04961 |
Applications
|
Applications |
Species |
Dilution |
Positive Sample |
|
WB |
Human |
1: 500-1: 2000 |
testis, colon |
|
IHC/IF |
Human, Mouse |
1: 400-1: 2000 |
lung cancer, ovarian cancer, testis |
Background
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.
Images
|
|
Western blot analysis of PCNA (GB11010-1) at dilution of 1: 500 |
|
|
Immunofluorescence of paraffin embedded human breast cancer using PCNA (GB11010-1) at dilution of 1: 400 (300x lens) |
|
|
Immunofluorescence of paraffin embedded human breast cancer using PCNA (GB11010-1) at dilution of 1: 400 (400x lens) |
|
|
Immunofluorescence of paraffin embedded mouse testis using PCNA (GB11010-1) at dilution of 1: 400 (250x lens) |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室