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Product Information
Description |
Cyclin B1 rabbit polyclonal |
Protein full name |
G2/mitotic-specific cyclin-B1 |
Synonyms |
Ccn-2, Ccnb1-rs13, Cycb, Cycb1, Ccnb1, CCNB |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Cyclin B1 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
|
Predicted MW. |
48 kDa |
Observed MW. |
55 kDa |
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
testis, colon, spleen |
IHC |
Human, Mouse, Rat |
1: 1500-1: 3000 |
colon cancer, skin, colon |
IF |
Human, Mouse, Rat |
1: 400-1: 800 |
lung cancer, cervical cancer, testis, colon |
Background
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Images
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Western blot analysis of Cyclin B1 (GB112098) at dilution of 1: 1000 Lane 1: Jurkat cell lysate Lane 2: 293T cell lysate Lane 3: Mouse testis tissue lysate Lane 4: Mouse colon tissue lysate Lane 5: Rat testis tissue lysate Lane 6: Rat colon tissue lysate Lane 7: Rat spleen tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded human colon cancer using Cyclin B1 (GB112098) at dilution of 1: 3000 |
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Immunohistochemistry analysis of paraffin-embedded human skin using Cyclin B1 (GB112098) at dilution of 1: 3000 |
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Immunohistochemistry analysis of paraffin-embedded mouse colon using Cyclin B1 (GB112098) at dilution of 1: 3000 |
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Immunohistochemistry analysis of paraffin-embedded rat colon using Cyclin B1 (GB112098) at dilution of 1: 3000 |
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Immunofluorescent analysis of paraformaldehyde-fixed human lung cancer using Cyclin B1 (GB112098) at dilution of 1: 800 |
|
Immunofluorescent analysis of paraformaldehyde-fixed human cervical cancer using Cyclin B1 (GB112098) at dilution of 1: 800 |
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using Cyclin B1 (GB112098) at dilution of 1: 800 |
|
Immunofluorescent analysis of paraformaldehyde-fixed rat testis using Cyclin B1 (GB112098) at dilution of 1: 800 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat colon using Cyclin B1 (GB112098) at dilution of 1: 800
|
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
合肥万物生物科技有限公司
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