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产品详情
操作说明
注意事项
Product Information
|
Description |
CD74 rabbit polyclonal |
|
Protein full name |
H-2 class II histocompatibility antigen gamma chain |
|
Synonyms |
Ia antigen-associated invariant chain, Ii, MHC class II-associated invariant chain, CD74 |
|
Immunogen |
Recombinant protein corresponding to Mouse CD74 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cell Membrane |
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
IHC |
Mouse, Rat |
1:1000-1:4000 |
lymphoid, spleen, immune spleen |
| IF | Mouse, Rat | 1:500-1:1000 |
|
Background
CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of or in association with MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells.
Images
|
|
Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD74 (GB111179) at dilution of 1: 2000 |
|
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Immunohistochemistry analysis of paraffin-embedded mouse immune spleen using CD74 (GB111179) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse lymphoid using CD74 (GB111179) at dilution of 1: 2000 |
|
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Immunohistochemistry analysis of paraffin-embedded rat lymphoid using CD74 (GB111179) at dilution of 1: 2000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室