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Anti -MCM2 Rabbit pAb

价格: 在线咨询
货号:
KT012100
产品规格:
100 μL
Applications:
WB/IHC/IF
Species:
HMR/HMR
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

MCM2 rabbit polyclonal antibody

Protein full name

DNA replication licensing factor MCM2

Synonyms

Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, Mcm2, Bm28, Cdcl1, Kiaa0030, Mcmd2

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse MCM2

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW.

102 kDa

Observed MW.

130 kDa

Uniprot ID

P49736, P97310, D3ZP96




Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 500-1: 1000

thymus

IHC/IF

Human, Mouse, Rat

1: 1000-1: 4000

brain, colon cancer, lung, lung cancer, thymus




Background


The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. 




Images


Picture 1.png

Western blot analysis of MCM2 (GB111162) at dilution of 1: 500

Picture 2.png

Immunohistochemistry of paraffin embedded human colon cancer using MCM2 (GB111162) at dilution of 1: 4000 (400x lens)

Picture 3.png

Immunohistochemistry of paraffin embedded human lung cancer using MCM2 (GB111162) at dilution of 1: 4000 (400x lens)

Picture 4.png

Immunohistochemistry of paraffin embedded mouse brain using MCM2 (GB111162) at dilution of 1: 2000 (400x lens)

Picture 5.png

Immunohistochemistry of paraffin embedded mouse thymus using MCM2 (GB111162) at dilution of 1: 4000 (400x lens)

Picture 6.png

Immunohistochemistry of paraffin embedded rat lung using MCM2 (GB111162) at dilution of 1: 2000 (400x lens)

Picture 7.png

Immunohistochemistry of paraffin embedded rat thymus using MCM2 (GB111162) at dilution of 1: 4000 (400x lens)

Picture 8.png

Immunofluorescence of paraffin embedded mouse brain using MCM2 (GB111162) at dilution of 1: 2000 (400x lens)

Picture 9.png

Immunofluorescence of paraffin embedded rat thymus using MCM2 (GB111162) at dilution of 1: 2000 (400x lens)




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).


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