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产品详情
操作说明
注意事项
Product Information
|
Description |
CD3 rabbit polyclonal |
|
Protein full name |
T-cell surface glycoprotein CD3 epsilon chain |
|
Synonyms |
CD3E, IMD18, T3E, TCRE, CD3e molecule |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse CD3 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted molecular |
23 kDa |
|
Observed molecular |
22 kDa |
|
Subcellular location |
Cell membrane |
|
Uniprot ID |
P22646 |
Applications
|
applications |
species |
dilution |
Positive tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
thymus, lymph node |
|
IHC |
Human, Mouse, Rat |
1: 700-1: 1400 |
lymph node, tonsil, spleen |
|
IF |
Human, Mouse, Rat |
1: 700-1: 1400 |
tonsil, spleen |
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
Images
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Western blot analysis of CD3 (GB111337) at dilution of 1: 1000 |
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Western blot analysis of CD3 (GB111337) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded human tonsil using CD3 (GB111337) at dilution of 1: 700 |
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Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD3 (GB111337) at dilution of 1: 700 |
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Immunohistochemistry analysis of paraffin-embedded rat spleen using CD3 (GB111337) at dilution of 1: 700 |
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Immunohistochemistry analysis of paraffin-embedded rat lymph node using CD3 (GB111337) at dilution of 1: 700 |
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Immunohistochemistry analysis of paraffin-embedded rat lymph using CD3 (GB111337) at dilution of 1: 1400 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse spleen using CD3 (GB111337) at dilution of 1: 700 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat spleen using CD3 (GB111337) at dilution of 1: 700 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室