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Anti -GAPDH Mouse mAb

价格: 在线咨询
货号:
16
产品规格:
100 μL
Applications:
WB | IHC | IF
Species:
H,M,R | H,M,R | H,M,R
购买数量:
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产品详情 操作说明 注意事项

Product Information


Description

GAPDH mouse monoclonal

Protein full name

Glyceraldehyde-3-phosphate dehydrogenase

Synonyms

GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse GAPDH

Isotype

IgG

Predicted MW.

37 kDa

Observed MW.

37 kDa

Subcellular location

CytoplasmNucleusMembrane

Purity

Affinity purification

Uniprot ID

P04406,P04797,P16858



Applications


applications

species

dilution

Positive tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

spleen, lung, kidney, brain, heart, skeletal muscle

IHC

Human, Mouse, Rat

1: 200-1: 400

stomach, testis, kidney

IF

Human, Mouse, Rat

1: 200-1: 400

stomach, testis, kidney



Background


Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.




Images



Western blot analysis of GAPDH (GB12002) at dilution of 1:1000

Western blot analysis of GAPDH (GB12002) at dilution of 1:1000


Immunohistochemistry analysis of paraffin-embedded human stomach using 

GAPDH (GB12002) at dilution of 1:200

image.png

Immunohistochemistry analysis of paraffin-embedded mouse testis using 

GAPDH (GB12002) at dilution of 1:200

image.png

Immunohistochemistry analysis of paraffin-embedded rat kidney using 

GAPDH (GB12002) at dilution of 1:200




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .



Publications:


Title

Journal

Pubmed ID

Application

1

Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells.

Aging (Albany NY)

33290258

WB

2

Hyperoside Attenuates Bleomycin-Induced Pulmonary Fibrosis Development in Mice.
Abstract

Frontiers in Pharmacology

33192501

WB

3

African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.

Virologica Sinica

33141406

WB

4

Bioinformatics analysis of LINC01554 and its co‑expressed genes in hepatocellular carcinoma.

Oncology Reports

33000250

WB

5

DEPDC1B promotes migration and invasion in pancreatic ductal adenocarcinoma by activating the Akt/GSK3β/Snail pathway.

Oncology Letters

32934714

WB

6

The tissue specific regulation of miR22 expression in the lung and brain by ribosomal protein L29.

Scientific Reports

33004906

WB

7

Protective effect of dimethyl fumarate on oxidative damage and signaling in cardiomyocytes.

Molecular Medicine Reports

32945364

WB

8

RNF181 modulates Hippo signaling and triple negative breast cancer progression.

Cancer Cell International

32655323

WB

9

A protein palmitoylation cascade regulates microtubule cytoskeleton integrity in Plasmodium.

The EMBO Journal

32395856

WB

10

Hypoxia/reoxygenation activates the JNK pathway and accelerates synovial senescence.

Molecular Medicine Reports

32377698

WB

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