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产品详情
操作说明
注意事项
Product Information
|
Description |
GAPDH rabbit polyclonal |
|
Protein full name |
Glyceraldehyde-3-phosphate dehydrogenase |
|
Synonyms |
GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse GAPDH |
|
Isotype |
IgG |
|
Predicted MW. |
37kDa |
|
Observed MW. |
37kDa |
|
Purity |
Affinity purification |
|
Uniprot ID |
Applications
|
applications |
species |
dilution |
Positive tissue |
|
WB |
Human, Mouse, Rat |
1:1000-1:2000 |
brain, spleen, kidney, liver |
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.
Images
|
|
Western blot analysis of GAPDH (GB11002) at dilution of 1: 2000 Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF-7 cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Rat kidney tissue lysate Lane 9: Rat brain tissue lysate |
|
|
Western blot analysis of GAPDH (GB11002) at dilution of 1: 2000 Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Rat kidney tissue lysate Lane 7: Rat brain tissue lysate |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
Publications:
|
|
Title |
Journal |
Pubmed ID |
Application |
|
1 |
Resveratrol alleviates oxygen/glucose deprivation/reoxygenation-induced neuronal damage through induction of mitophagy. |
Molecular Medicine Reports |
33236158 |
WB |
|
2 |
Deubiquitinase USP7 regulates Drosophila aging through ubiquitination and autophagy. |
Aging (Albany NY) |
33221768 |
WB |
|
3 |
Epigallocatechin gallate induces chemopreventive effects on rats with diethylnitrosamine-induced liver cancer via inhibition of cell division cycle 25A. |
Molecular Medicine Reports |
33000276 |
WB |
|
4 |
Xiaoyao Pills Attenuate Inflammation and Nerve Injury Induced by Lipopolysaccharide in Hippocampal Neurons In Vitro. |
Neural Plasticity |
33014035 |
WB |
|
5 |
PEDF promotes the repair of bone marrow endothelial cell injury and accelerates hematopoietic reconstruction after bone marrow transplantation. |
Journal of Biomedical Science |
32873283 |
WB |
|
6 |
Telomerase inhibition decreases esophageal squamous carcinoma cell migration and invasion. |
Oncology Letters |
32782603 |
WB |
|
7 |
Dihydrolipoic acid protects against lipopolysaccharide-induced behavioral deficits and neuroinflammation via regulation of Nrf2/HO-1/NLRP3 signaling in rat. |
Journal of Neuroinflammation |
32450903 |
WB |
|
8 |
5-Aza-2'-deoxycytidine advances the epithelial-mesenchymal transition of breast cancer cells by demethylating Sipa1 promoter-proximal elements. |
Journal of Cell Science |
32193333 |
WB |
|
9 |
Effect of cold stress on ovarian & uterine microcirculation in rats and the role of endothelin system. |
Reproductive Biology and Endocrinology : RB&E |
32290862 |
WB |
|
10 |
Xiaoyao Pills Prevent Lipopolysaccharide-Induced Depression by Inhibiting Inflammation and Protecting Nerves. |
Frontiers in Pharmacology |
31798446 |
WB |
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客服热线：400-1016-218
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地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室