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Anti -GAPDH Mouse mAb

价格: 在线咨询
货号:
DRF10112
产品规格:
100 μL
Applications:
WB
Species:
H,M,R
购买数量:
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产品详情 操作说明 注意事项

Product Information


Description

alpha Actinin rabbit polyclonal

Protein full name

Alpha-actinin-1

Synonyms

Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, actinin, Non-muscle alpha-actinin-1, ACTN1, actinin 1 smooth muscle, Actn1a, BDPLT15, I79_004057

Immunogen

Recombinant protein corresponding to Mouse α-actinin1

Isotype

IgG

Purity

Affinity purification

Predicted MW.

103 kDa

Observed MW.

103 kDa

Uniprot ID

Q7TPR4, P12814, Q9Z1P2




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 2000-1: 4000

lung, stomach, colon, hysteromyoma




Background


Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found alongmicrofilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.

 



Images



图片 1.png

Western blot analysis of α-actinin (GB11230) at dilution of 1: 2000.

Lane 1: HepG2 cell lysate

Lane 2: AN3CA cell lysate

Lane 3: Human hysteromyoma tissue lysate

Lane 4: Mouse lung tissue lysate

Lane 5: Mouse stomach tissue lysate

Lane 6: Mouse colon tissue lysate

Lane 7: Rat lung tissue lysate

Lane 8: Rat stomach tissue lysate

Lane 9: Rat colon tissue lysate




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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