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Anti -EIF2S2/EIF2B Rabbit pAb

价格: 在线咨询
货号:
KT012119
产品规格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M/R,H/M/R,H/M/R
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

EIF2S2 rabbit polyclonal

Protein full name

Eukaryotic translation initiation factor 2 subunit 2

Synonyms

eIF-2-beta, Eif2s2, Eukaryotic translation initiation factor 2 subunit beta

Immunogen

Recombinant protein corresponding to Mouse EIF2S2

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

38 kDa

Observed MW.

50 kDa

Uniprot ID

P20042, Q99L45




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

lung, colon, small intestine

IHC

Human, Mouse, Rat

1: 1000-1: 2000

colon, lung, placenta

IF

Human, Mouse, Rat

1: 500-1: 3000

colon, brain




Background


Eukaryotic translation initiation factor 2 (eIF2) is composed of three subunits, eIF2 alpha, eIF2 beta (EIF2S2), and eIF2 gamma, which are present in equal molar amounts. eIF2 beta plays a central role in the maintenance of what is generally considered a rate-limiting step in mRNA translation. In the early steps of protein synthesis, eIF2 beta binds GTP and Met-tRNA and transfers Met-tRNA to the 40S ribosomal subunit. At the end of the initiation process, GTP bound to eIF2 beta is hydrolyzed to GDP and the eIF2/GDP complex is released from the ribosome. The exchange of GDP bound to eIF2 beta for GTP is a prerequisite to binding Met-tRNA and is mediated by eIF2 beta, which recycles the eIF2 complex for another round of initiation.




Images


Western blot analysis of EIF2S2 (GB111135) at dilution of 1: 1000
Lane 1: HT29 cell lysate
Lane 2: L1299 cell lysate
Lane 3: CaCO2 cell lysate
Lane 4: Mouse lung tissue lysate
Lane 5: Mouse colon tissue lysate
Lane 6: Mouse small intestine tissue lysate
Lane 7: Rat lung tissue lysate

Immunohistochemistry analysis of paraffin-embedded human colon using EIF2S2 (GB111135) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded rat lung using EIF2S2 (GB111135) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat placenta using EIF2S2 (GB111135) at dilution of 1: 1000

Immunofluorescent analysis of paraffin-embedded human colon using EIF2S2 (GB111135) at dilution of 1: 1000

Immunofluorescent analysis of paraffin-embedded rat brain using EIF2S2 (GB111135) at dilution of 1: 1000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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