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Anti -Nucleophosmin Rabbit pAb

价格: 在线咨询
货号:
KT012085
产品规格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M/R,H/M/R,H/M/R
购买数量:
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产品详情 操作说明 注意事项

Product Information


Description

Nucleophosmin rabbit polyclonal antibody

Protein full name

Nucleophosmin

Synonyms

NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, Npm1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Nucleophosmin

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW.

33 kDa

Observed MW.

40 kDa

Uniprot ID

P06748, Q61937, P13084




Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 500-1: 1000

spleen, testis

IHC/IF

Human, Mouse, Rat

1: 500-1: 3000

breast cancer, colon, colon cancer, hepatocarcinoma, lung cancer, lymph node, ovarian cancer, ovary, spleen, testis




Background


Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation and regulation of tumor suppressors p53/TP53 and ARF. Stimulates APEX1endonuclease activity on apurinic/apyrimidinic double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules.




Images


Picture 1.png

Western blot analysis of Nucleophosmin (GB111043) at dilution of 1: 500

Picture 2.png Immunohistochemistry of paraffin embedded human colon cancer using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)
Picture 3.png Immunohistochemistry of paraffin embedded human hepatocarcinoma using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)
Picture 4.png Immunohistochemistry of paraffin embedded human lung cancer using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)
Picture 5.png Immunohistochemistry of paraffin embedded human ovarian cancer using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)
Picture 6.png Immunohistochemistry of paraffin embedded mouse colon using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)

Picture 7.png

Immunohistochemistry of paraffin embedded mouse lymph node using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)

Picture 8.png

Immunohistochemistry of paraffin embedded mouse ovary using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)

Picture 9.png

Immunohistochemistry of paraffin embedded mouse spleen using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)

Picture 10.png

Immunohistochemistry of paraffin embedded mouse testis using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)

Picture 11.png

Immunohistochemistry of paraffin embedded rat colon using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)

Picture 12.png

Immunohistochemistry of paraffin embedded rat lymph node using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)

Picture 13.png

Immunohistochemistry of paraffin embedded rat ovary using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)

Picture 14.png

Immunohistochemistry of paraffin embedded rat spleen using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)

Picture 15.png

Immunohistochemistry of paraffin embedded rat testis using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens)

Picture 16.png

Immunofluorescence of paraffin embedded human breast cancer using Nucleophosmin (GB111043) at dilution of 1: 1400 (400x lens)

Picture 17.png

Immunofluorescence of paraffin embedded human ovarian cancer using Nucleophosmin (GB111043) at dilution of 1: 1400 (400x lens)

Picture 18.png

Immunofluorescence of paraffin embedded rat spleen using Nucleophosmin (GB111043) at dilution of 1: 1400 (400x lens)

Picture 19.png

Immunofluorescence of paraffin-embedded rat testis using Nucleophosmin (GB111043) at dilution of 1: 1400 (400x lens)




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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