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Product Information
Description |
Nucleophosmin rabbit polyclonal antibody |
Protein full name |
Nucleophosmin |
Synonyms |
NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, Npm1 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Nucleophosmin |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Nucleus, Cytoplasm |
Predicted MW. |
33 kDa |
Observed MW. |
40 kDa |
Uniprot ID |
P06748, Q61937, P13084 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
spleen, testis |
IHC/IF |
Human, Mouse, Rat |
1: 500-1: 3000 |
breast cancer, colon, colon cancer, hepatocarcinoma, lung cancer, lymph node, ovarian cancer, ovary, spleen, testis |
Background
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation and regulation of tumor suppressors p53/TP53 and ARF. Stimulates APEX1endonuclease activity on apurinic/apyrimidinic double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules.
Images
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Western blot analysis of Nucleophosmin (GB111043) at dilution of 1: 500 |
Immunohistochemistry of paraffin embedded human colon cancer using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) | |
Immunohistochemistry of paraffin embedded human hepatocarcinoma using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) | |
Immunohistochemistry of paraffin embedded human lung cancer using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) | |
Immunohistochemistry of paraffin embedded human ovarian cancer using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) | |
Immunohistochemistry of paraffin embedded mouse colon using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) | |
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Immunohistochemistry of paraffin embedded mouse lymph node using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse ovary using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse spleen using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse testis using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat colon using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat lymph node using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat ovary using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) |
|
Immunohistochemistry of paraffin embedded rat spleen using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) |
|
Immunohistochemistry of paraffin embedded rat testis using Nucleophosmin (GB111043) at dilution of 1: 3000 (400x lens) |
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Immunofluorescence of paraffin embedded human breast cancer using Nucleophosmin (GB111043) at dilution of 1: 1400 (400x lens) |
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Immunofluorescence of paraffin embedded human ovarian cancer using Nucleophosmin (GB111043) at dilution of 1: 1400 (400x lens) |
|
Immunofluorescence of paraffin embedded rat spleen using Nucleophosmin (GB111043) at dilution of 1: 1400 (400x lens) |
|
Immunofluorescence of paraffin-embedded rat testis using Nucleophosmin (GB111043) at dilution of 1: 1400 (400x lens) |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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