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产品详情
操作说明
注意事项
Product Information
|
Description |
Hsp27 rabbit polyclonal antibody |
|
Protein full name |
Heat shock protein beta-1 |
|
Synonyms |
HspB1, Estrogen-regulated 24 kDa protein, 28 kDa heat shock protein, Heat shock 27 kDa protein, HSP27, Stress-responsive protein 27, SRP27, HSP28 |
|
Immunogen |
Recombinant protein corresponding to Human Hsp27 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Nucleus, Cytoplasm |
|
Predicted MW. |
23 kDa |
|
Observed MW. |
27 kDa |
|
Uniprot ID |
P04792 |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human |
1: 10000-1: 20000 |
HeLa, MCF7, A549, SH-SY5Y, SKOV3 |
|
IHC/IF |
Human |
1: 1000-1: 3000 |
breast cancer, lung cancer
|
Background
HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma.
Images
|
|
Western blot analysis of HSP27 (GB111176) at dilution of 1: 10000 |
|
|
Immunohistochemistry of paraffin embedded human breast cancer using HSP27 (GB111176) at dilution of 1: 1500 (400x lens) |
|
|
Immunohistochemistry of paraffin embedded human lung cancer using HSP27 (GB111176) at dilution of 1: 1500 (400x lens) |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室