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Anti -Hsp27 Rabbit pAb

价格: 在线咨询
货号:
KT012058
产品规格:
Applications:
WB | IHC | IF
Species:
H/H
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

Hsp27 rabbit polyclonal antibody

Protein full name

Heat shock protein beta-1

Synonyms

HspB1, Estrogen-regulated 24 kDa protein, 28 kDa heat shock protein, Heat shock 27 kDa protein, HSP27, Stress-responsive protein 27, SRP27, HSP28

Immunogen

Recombinant protein corresponding to Human Hsp27

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW.

23 kDa

Observed MW.

27 kDa

Uniprot ID

P04792




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human

1: 10000-1: 20000

HeLa, MCF7, A549, SH-SY5Y, SKOV3

IHC/IF

Human

1: 1000-1: 3000

breast cancer, lung cancer





Background


HSPB1, also known as heat shock protein 27 (Hsp27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma.




Images


Picture 1.png

Western blot analysis of HSP27 (GB111176) at dilution of 1: 10000

Picture 2.png

Immunohistochemistry of paraffin embedded human breast cancer using HSP27 (GB111176) at dilution of 1: 1500 (400x lens)

Picture 3.png

Immunohistochemistry of paraffin embedded human lung cancer using HSP27 (GB111176) at dilution of 1: 1500 (400x lens)




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).



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