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产品详情
操作说明
注意事项
Product Information
|
Description |
IDH1 rabbit polyclonal antibody |
|
Protein full name |
Isocitrate dehydrogenase [NADP] cytoplasmic |
|
Synonyms |
Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH1, Idh1 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse IDH1 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. |
47 kDa |
|
Observed MW. |
47 kDa |
|
Uniprot ID |
O75874, O88844, P41562 |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
liver, kidney, stomach |
Background
The Isocitrate dehydrogenase cytoplasmic enzyme belongs to the isocitrate and isopropylmalate dehydrogenases family. IDHC catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming ketoglutarate and CO2 in a two step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Ca2+ can bind to IDHC as a complex with isocitrate, acting as a competitive inhibitor of Mg2+. The IDHC enzyme is inactivated by phosphorylation at Ser-113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock.
Images
|
|
Western blot analysis of IDH1 (GB111194) at dilution of 1: 500 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室