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Anti -Heme-regulated inhibitor Rabbit pAb

价格: 在线咨询
货号:
KT012021
产品规格:
100ul
Applications:
WB/IHC/IF
Species:
H/M/R,M/R,M/R
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

Heme-regulated inhibitor rabbit polyclonal

Protein full name

Eukaryotic translation initiation factor 2-alpha kinase 1

Synonyms

Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Eif2ak1, Heme-controlled repressor, HCR, Heme-regulated inhibitor, Hri

Immunogen

Recombinant protein corresponding to Mouse Heme-regulated inhibitor

Isotype

IgG

Purity

Affinity purification

Predicted MW.

70 kDa

Observed MW.

70 kDa

Subcellular location

Cytoplasm

Uniprot ID

Q9BQI3Q9Z2R9Q63185




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

HEK293

IHC

Mouse, Rat

1: 800-1: 1600

bone, lung, inflammation lung

IF

Mouse, Rat

1: 500-1: 1000

bone, lung, inflammation lung




Background


The Eif2ak1 Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells, controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties.




Images


Western blot analysis of Eif2ak1  (GB111094) at dilution of 1: 1000

Lane 1: HEK293 cell lysate

Immunohistochemistry analysis of paraffin-embedded mouse bone using Eif2ak1 (GB111094) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded rat lung using Eif2ak1 (GB111094) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded mouse inflammation lung using Eif2ak1 (GB111094) at dilution of 1: 1000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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