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Product Information
Description |
COX IV mouse monoclonal |
Protein full name |
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
Synonyms |
Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cox4i1, Cox4, Cox4a |
Immunogen |
Recombinant protein corresponding to Mouse COX IV |
Isotype |
IgG2a |
Purity |
Affinity purification |
Subcellular location |
Mitochondrion inner membrane |
Predicted MW. |
20 kDa |
Observed MW. |
17 kDa |
Uniprot ID |
P13073, P19783, P10888 |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Mouse, Rat |
1: 1000-1: 2000 |
brain, heart, kidney, liver |
IHC |
Mouse, Rat |
1: 1000-1: 2000 |
brain, heart, kidney, liver |
Background
COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.
Images
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Western blot analysis of COX4 (GB12250) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse brain using COX4 (GB12250) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse heart using COX4 (GB12250) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse kidney using COX4 (GB12250) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat heart using COX4 (GB12250) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat kidney using COX4 (GB12250) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat liver using COX4 (GB12250) at dilution of 1: 2000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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