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Product Information
Description |
Cytochrome C mouse monoclonal |
Protein full name |
Cytochrome c |
Synonyms |
CYCS, CYC, HCS, THC4, cytochrome c, somatic, Cytochrome c, cyt c |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Cytochrome c |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. |
12 kDa |
Observed MW. |
14 KDa |
Subcellular location |
Mitochondrion intermembrane space |
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Mouse, Rat |
1: 500-1: 1000 |
heart |
IHC |
Human, Rat |
1: 500-1: 1000 |
stomach, heart |
IF |
Human, Rat |
1: 250-1: 1000 |
stomach, heart |
Background
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase.Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol.
Images
Western blot analysis of Cytochrome c (GB12080) at dilution of 1: 1000 .
Lane 1: Mouse heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lane 4: Rat heart tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded human stomach using Cytochrome c (GB12080) at dilution of 1: 1000 |
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Immunofluorescence analysis of paraffin-embedded rat heart using Cytochrome c (GB12080) at dilution of 1: 1000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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