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Product Information
Description |
Villin mouse molyclonal |
Protein full name |
Villin-1 |
Synonyms |
VIL1, VIL, Villin 1, D2S1471 |
Immunogen |
Recombinant protein corresponding to Mouse Villin |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
|
Predicted MW. |
93 kDa |
Observed MW. |
95 kDa |
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
small intestine, kidney, colon |
IHC |
Human, Mouse, Rat |
1: 500-1: 2000 |
small intestine, jejunum |
IF | Human, Mouse, Rat | 1: 500-1: 1000 |
|
Background
Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma.
Images
|
Western blot analysis of Villin (GB121209) at dilution of 1: 1000 Lane 1: CaCO2 cell lysate Lane 2: Mouse small intestine tissue lysate Lane 3: Mouse kidney tissue lysate Lane 4: Mouse colon tissue lysate Lane 5: Rat small intestine tissue lysate Lane 6: Rat kidney tissue lysate Lane 7: Rat colon tissue lysate |
|
Immunohistochemistry analysis of paraffin-embedded human small intestine using Villin (GB121209) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse jejunum using Villin (GB121209) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded rat jejunum using Villin (GB121209) at dilution of 1: 2000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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