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Product Information
Description |
GRP78 BiP mouse monoclonal |
Protein full name |
78 kDa glucose-regulated protein |
Synonyms |
HSPA5, BIP, GRP78, HEL-S-89n, MIF2, Binding immunoglobulin protein, heat shock protein family A (Hsp70) member 5 |
Immunogen |
Recombinant protein corresponding to Mouse GRP78 BiP |
Isotype |
IgG2a |
Purity |
Affinity purification |
Subcellular location |
|
Predicted MW. |
78 kDa |
Observed MW. |
78 kDa |
Uniprot ID |
P11021, P20029, P06761 |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
lung, kidney, colon |
IHC |
Human, Mouse, Rat |
1: 1000-1: 2000 |
liver cancer, brain, kidney |
IF |
Human, Mouse, Rat |
1: 1000-1: 2000 |
breast cancer, liver cancer, brain, kidney, colon |
Background
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Images
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Western blot analysis of BiP (GB12098) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded human liver cancer using BiP (GB12098) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse brain using BiP (GB12098) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat kidney using BiP (GB12098) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed human breast cancer using BiP (GB12098) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed human liver cancer using BiP (GB12098) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using BiP (GB12098) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat brain using BiP (GB12098) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse kidney using BiP (GB12098) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat kidney using BiP (GB12098) at dilution of 1: 2000 |
Immunofluorescent analysis of paraformaldehyde-fixed rat colon using BiP (GB12098) at dilution of 1: 2000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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