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产品详情
操作说明
注意事项
Product Information
| Description |
Desmin mouse monoclonal |
| Protein full name | Desmin |
|
Synonyms |
DES, CSM1, CSM2, LGMD2R, desmin |
| Immunogen |
Recombinant protein corresponding to Mouse desmin |
| Isotype | IgG |
| Purity | Affinity purification |
| Predicted MW. |
54 kDa |
| Observed MW. |
54 kDa |
| Subcellular location |
Cytoplasm,Cell membrane |
| Uniprot ID | P31001,P48675 |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Mouse, Rat |
1: 1000-1: 2000 |
Heart,C2C12 cell |
|
IHC |
Mouse, Rat |
1: 200-1: 1000 |
Heart,skeletal muscle |
|
IF |
Mouse, Rat |
1: 200-1: 1000 |
Heart,skeletal muscle |
Background
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures . May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction.
Images
|
|
Western blot analysis of Desmin (GB12081) at dilution of 1:1000. Lane 1:C2C12 cell lysate Lane 2:Hela cell lysate Lane 3:Raw264.7 cell lysate Lane 4:Mouse heart tissue lysate Lane 5:Mouse spleen tissue lysate Lane 6:Mouse lung tissue lysate Lane 7:Rat heart tissue lysate |
|
Immunohistochemistry analysis of parafffin-embedded mouse heart using Desmin (GB12081) at dilution of 1:500 |
|
Immunohistochemistry analysis of parafffin-embedded rat skeletal muscle using Desmin (GB12081) at dilution of 1:500 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
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地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室