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Product Information
Description |
PCNA mouse monoclonal |
Protein full name |
Proliferating cell nuclear antigen |
Synonyms |
PCNA, ATLD2, proliferating cell nuclear antigen |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse PCNA |
Isotype |
IgG |
Purity |
|
Subcellular location |
29 kDa |
Predicted MW. |
36 kDa |
Observed MW. |
Affinity purification |
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
testis, placenta |
IHC |
Mouse, Rat |
1: 2000-1: 4000 |
testis, colon |
Background
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.
Images
|
Western blot analysis of PCNA (GB12010) at dilution of 1: 1000 Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A549 cell lysate Lane 4: NIH3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Mouse placenta tissue lysate Lane 8: Rat testis tissue lysate Lane 9: Rat placenta tissue lysate |
|
Immunohistochemistry analysis of paraffin-embedded mouse testis using PCNA (GB12010) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded rat testis using PCNA (GB12010) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse colon using PCNA (GB12010) at dilution of 1: 2000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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