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Product Information
Description |
beta Actin mouse monoclonal |
Protein full name |
Actin, cytoplasmic 1 |
Synonyms |
ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse β-Actin |
Isotype |
IgG |
Predicted MW. |
42 kDa |
Observed MW. |
45 kDa |
Purity |
Affinity purification |
Subcellular location |
|
Uniprot ID |
P60709, P60710, P60711 |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Rat, Mouse |
1: 1000-1: 2000 |
kidney, brain, spleen |
IHC |
Human, Rat, Mouse |
1: 400-1: 2000 |
para-cancer colon, lung, stomach, liver cancer |
IF |
Human, Rat, Mouse |
1: 400-1: 2000 |
para-cancer colon, lung, stomach, liver cancer, |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
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Western blot analysis of β-Actin(GB12001) at dilution of 1: 1000 |
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Western blot analysis of β-Actin(GB12001) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded human liver cancer using β-Actin(GB12001) at dilution of 1:400 |
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Immunohistochemistry analysis of paraffin-embedded human para-cancer colon using β-Actin(GB12001) at dilution of 1:400 |
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Immunohistochemistry analysis of paraffin-embedded mouse stomach using β-Actin(GB12001) at dilution of 1:400 |
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Immunohistochemistry analysis of paraffin-embedded rat liver using β-Actin(GB12001) at dilution of 1:400 |
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Immunohistochemistry analysis of paraffin-embedded rat kidney using β-Actin(GB12001) at dilution of 1:400 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
Publications:
|
Title |
Journal |
Pubmed ID |
Application |
1 |
Time-Restricted Feeding Reduces the Detrimental Effects of a High-Fat Diet, Possibly by Modulating the Circadian Rhythm of Hepatic Lipid Metabolism and Gut Microbiota. |
Frontiers in Nutrition |
33425971 |
WB |
2 |
Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis. |
Oncology Letters |
33029205 |
WB |
3 |
TRIM3 inhibits P53 signaling in breast cancer cells. |
Cancer Cell International |
33292295 |
WB |
4 |
CAB39 Promotes the Proliferation of Nasopharyngeal Carcinoma CNE-1 Cells via Up-Regulating p-JNK. |
Cancer Management and Research |
33177871 |
WB |
5 |
MicroRNA23a5p mediates the proliferation and differentiation of C2C12 myoblasts. |
Molecular Medicine Reports |
32901860 |
WB |
6 |
NLRC5 Inhibits Inflammation of Secretory Phase Ectopic Endometrial Stromal Cells by Up-Regulating Autophagy in Ovarian Endometriosis. |
Frontiers in Pharmacology |
33013364 |
WB |
7 |
RACO-1 modulates Hippo signalling in oesophageal squamous cell carcinoma. |
Journal of Cellular and Molecular Medicine |
32896069 |
WB |
8 |
CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNA. |
BMC Biology |
32887607 |
WB |
9 |
Synergistic Effects of Ginkgolide B and Protocatechuic Acid on the Treatment of Parkinson's Disease. |
Molecules (Basel, Switzerland) |
32878312 |
WB |
10 |
Effect of Moxibustion on the Intestinal Flora of Rats with Knee Osteoarthritis Induced by Monosodium Iodoacetate. |
Evidence-based Complementary and Alternative Medicine : eCAM |
32714401 |
WB |
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