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产品详情
操作说明
注意事项
Product Information
|
Description |
Rab5 rabbit polyclonal |
|
Protein full name |
Ras-related protein Rab-5A |
|
Synonyms |
Rab5a, NnyRab5a, Ubiquitin-protein ligase D3 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Human Rab5 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. |
24 kDa |
|
Observed MW. |
24 kDa |
|
Subcellular location |
|
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
brain, testis |
|
IHC |
Human |
1: 1000-1: 2000 |
lung, lung cancer |
|
IF |
Human |
1: 500-1: 1000 |
lung, lung cancer |
Background
Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes, and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nucleotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. The GTP-bound, or active form of Rab5 associates with membrane s and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolyzed xanthosine 5-triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 may determine the frequency of membrane docking and fusion events.
Images
|
|
Western blot analysis of Rab5 (GB111104) at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embeddehuman lung using Rab5 (GB111104) at dilution of 1: 2000 |
|
|
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Rab5 (GB111104) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室