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Anti -HMGB1 Rabbit pAb
价格：
在线咨询
货号：
DRF101146
产品规格：
100 μL
Applications：
WB | IHC | IF
Species：
H,M,R | H,M,R | H,M,R
购买数量：
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400-1016-218
产品详情
操作说明
注意事项
Product Information
|
Description |
HMGB1 rabbit polyclonal |
|
Protein full name |
High mobility group protein B1 |
|
Synonyms |
HMGB1, HMG1, HMG3, SBP-1, HMG-1, high mobility group box 1 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse HMGB1 |
|
Isotype |
IgG |
|
Predicted MW. |
25KDa |
|
Observed MW. |
25KDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Nucleus, Cytoplasm, Secreted, Cell membrane |
|
Uniprot ID |
Applications
|
applications |
species |
dilution |
Positive tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
nasopharyngeal carcinoma, liver, cancer, kidney, colon cancer |
|
IHC |
Human, Mouse, Rat |
1: 300-1: 3000 |
colon cancer, breast cancer, brain, kidney, skin, spinal cord |
|
IF |
Human, Mouse, Rat |
1: 300-1: 1000 |
colon cancer, breast cancer, brain, kidney, skin, spinal cord |
Background
Like the histones, HMGB1 is among the most important chromatin proteins. In the nucleus HMGB1 interacts withnucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription.After binding, HMGB1 bendsDNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in interactions with many transcription factors. It also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. Contact with core histones changes the structure of nucleosomes.
Images
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Western blot analysis of HMGB1 (GB11103) at dilution of 1: 1000 Lane 1: HeLa cell lysate Lane 2: SW480 cell lysate Lane 3: B16 cell lysate Lane 4: Human nasopharyngeal carcinoma tissue lysate Lane 5: Human liver cancer tissue lysate Lane 6: Human colon cancer tissue lysate Lane 7: Mouse kidney tissue lysate Lane 8: Rat kidney tissue lysate |
|
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Immunohistochemistry analysis of paraffin-embedded human colon cancer HMGB1 (GB11103) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded human breast cancer HMGB1 (GB11103) at dilution of 1: 1000
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Immunohistochemistry analysis of paraffin-embedded human brain HMGB1 (GB11103) at dilution of 1: 1000 |
|
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Immunohistochemistry analysis of paraffin-embedded mouse kidney HMGB1 (GB11103) at dilution of 1: 1000 |
|
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Immunohistochemistry analysis of paraffin-embedded rat skin HMGB1 (GB11103) at dilution of 1: 1000 |
|
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Immunohistochemistry analysis of paraffin-embedded rat spinal cord HMGB1 (GB11103) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室