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Product Information
Description |
Collagen V rabbit polyclonal |
Protein full name |
Collagen alpha-1(V) chain |
Synonyms |
Col5a1, Collagen Type V, Alpha 1 type V collagen, Col5A2, Col5A3, Collagen alpha 2(V) chain, Collagen type V alpha 1 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Collagen V |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Extracellular region or secreted |
Predicted MW. |
184 kDa |
Observed MW. |
184 kDa |
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Mouse, Rat |
1: 500-1: 1000 |
liver fibrosis |
IHC |
Mouse, Rat |
1: 1500-1: 3000 |
skin, heart |
IF |
Mouse, Rat |
1: 750-1: 1500 |
skin, heart |
Background
COL5A1 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A1 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.
Images
Western blot analysis of Collagen V (GB111012) at dilution of 1: 1000 Lane 1: Mouse liver fibrosis tissue lysate |
|
Immunohistochemistry analysis of paraffin-embedded mouse skin using Collagen V (GB111012) at dilution of 1: 1800 |
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Immunohistochemistry analysis of paraffin-embedded mouse heart using Collagen V (GB111012) at dilution of 1: 1800 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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