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Product Information
Description |
GFAP rabbit polyclonal |
Protein full name |
Glial fibrillary acidic protein |
Synonyms |
GFAP, ALXDRD, glial fibrillary acidic protein, Astrocyte, Intermediate filament protein |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse GFAP |
Isotype |
IgG |
Predicted MW. |
49 kDa |
Observed MW. |
49 kDa |
Purity |
Affinity purification |
Subcellular location |
|
Uniprot ID |
Applications
applications |
species |
dilution |
Positive tissue |
WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
brain, cerebellum, cortex |
IHC |
Mouse, Rat |
1: 1000-1: 2000 |
brain, cerebellum |
IF |
Mouse, Rat |
1: 500-1: 3000 |
brain, hippocampus |
Background
Glial fibrillary acidic protein is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells.GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. It is closely related to its non-epithelial family members, vimentin,desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton.
Images
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Western blot analysis of GFAP (GB11096) at dilution of 1: 2000 |
Immunohistochemistry analysis of paraffin-embedded mouse brain using GFAP (GB11096) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat brain using GFAP (GB11096) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat cerebellum using GFAP (GB11096) at dilution of 1: 2000
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Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using GFAP (GB11096) at dilution of 1: 1000
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Immunofluorescent analysis of paraformaldehyde-fixed rat brain using GFAP (GB11096) at dilution of 1: 1000
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Immunofluorescent analysis of paraformaldehyde-fixed rat hippocampus using Neun (GB11138) (green) + IBA-1 (GB12105) (red) + MBP (GB12226) (spred) +GFAP (GB11096) (yellow) and DAPI in blue at dilution of 1: 1000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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