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Product Information
Description |
CD3 rabbit polyclonal |
Protein full name |
T-cell surface glycoprotein CD3 epsilon chain |
Synonyms |
CD3E, IMD18, T3E, TCRE, CD3e molecule |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse CD3 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Cell membrane |
Uniprot ID |
P07766, P22646 |
Applications
applications |
species |
dilution |
Positive tissue |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
spleen, lung, thymus, |
IHC |
Human, Mouse, Rat |
1: 250-1: 1000 |
tonsil, spleen |
IF |
Human, Mouse, Rat |
1: 250-1: 1000 |
tonsil, spleen |
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
Images
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Western blot analysis of CD3 (GB11014) at dilution of 1:1000. Lane 1:SH-SY5Y cell lysate Lane 2:THP-1 cell lysate Lane 3:Raw264.7 cell lysate Lane 4:Mouse spleen tissue lysate Lane 5:Mouse lung tissue lysate Lane 6: Mouse thymus tissue lysate Lane 7:Rat spleen tissue lysate Lane 8:Rat lung tissue lysate Lane 9:Rat thymus tissue lysate
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Immunohistochemistry analysis of paraffin-embedded human tonsil using CD3 (GB11014) at dilution of 1:500 (Note: pH6.0 citrate buffer high temperature -pressure repairing)
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Immunohistochemistry analysis of paraffin-embedded mouse Inflammatory spleen using CD3 (GB11014) at dilution of 1:500 (Note: pH6.0 citrate buffer high temperature -pressure repairing)
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Immunohistochemistry analysis of paraffin-embedded rat spleen using CD3 (GB11014) at dilution of 1:500 (Note: pH6.0 citrate buffer high temperature -pressure repairing)
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Immunohistochemistry analysis of paraffin-embedded rat Inflammatory spleen using CD3 (GB11014) at dilution of 1:250 (Note: pH6.0 citrate buffer high temperature -pressure repairing)
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Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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