服务热线
400-1016-218
联系方式
- 手机：400-1016-218
- Q Q：355185756
- 邮箱：355185756@qq.com
- 地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室
Anti -PCNA Rabbit pAb
价格：
在线咨询
货号：
DRF10151
产品规格：
100 μL
Applications：
WB/IHC/IF
Species：
H,H/M,H/M
购买数量：
在线咨询
400-1016-218
产品详情
操作说明
注意事项
Product Information
|
Description |
PCNA rabbit polyclonal |
|
Protein full name |
Proliferating cell nuclear antigen |
|
Synonyms |
PCNA, ATLD2, proliferating cell nuclear antige |
|
Immunogen |
Recombinant protein corresponding to Mouse PCNA |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. |
29 kDa |
|
Observed MW. |
36 kDa |
|
Subcellular location |
Nucleus |
|
Uniprot ID |
Applications
|
applications |
species |
dilution |
Positive tissue |
|
WB |
Human |
1: 500-1: 2000 |
HeLa, HepG2, MCF7, liver cancer |
|
IHC |
Human, Mouse |
1: 1000-1: 2000 |
lung cancer, heart |
|
IF |
Human, Mouse |
1: 400-1: 2000 |
lung cancer, testis, ovary cancer |
Background
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.
Images
|
|
Western blot analysis of PCNA (GB11010-1) at dilution of 1: 1000 Lane 1: HeLa cell lysate Lane 2: MCF-7 cell lysate Lane 3: 293T cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Mouse colon tissue lysate Lane 8: Rat testis tissue lysate Lane 9: Rat colon tissue lysate |
|
|
Immunohistochemistry analysis of paraffin-embedded human lung cancer using PCNA (GB11010-1) at dilution of 1: 2000 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse heart using PCNA (GB11010-1) at dilution of 1: 2000 |
|
|
Immunofluorescent analysis of paraformaldehyde-fixed human lung cancer using PCNA (GB11010-1) at dilution of 1: 2000 |
|
|
Immunofluorescent analysis of paraformaldehyde-fixed human ovary cancer using PCNA (GB11010-1) at dilution of 1: 2000
|
|
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using PCNA (GB11010-1) at dilution of 1: 2000
|
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
凡合肥万物生物科技有限公司销售的产品均有严格的质量保证。如发现产品存在包装规格疑问，请于收货之日起七日内向我公司总部或当地办事处提出，并请保证该产品，以备本公司进行核对检验。如果发现有质量问题，请于收货后一月内保留产品50%以上的量，以便本公司回收产品进行质检，确认产品质量。如逾期，则视为已经对本公司产品进行验收。 声明：如发生产品索赔事宜，本公司仅在产品价格范围内酌情赔偿，恕不接受超出产品价格范围之赔偿。客户在使用过程中有任何技术问题可以拨打技术售后服务电话我们随时给予解答。
合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室