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Anti - Flag Tag ( DYKDDDDK Tag ) Rabbit pAb

价格: 在线咨询
货号:
DRF10130
产品规格:
100 μL
Applications:
WB
Species:
--
购买数量:
在线咨询 400-1016-218
产品详情 操作说明 注意事项

Product Information


Description

Flag Tag ( DYKDDDDK Tag ) rabbit polyclonal 

Synonyms

DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG

Immunogen

KLH conjugated Synthetic peptide

corresponding to DYKDDDDK

Isotype

IgG

Purity

Affinity purification



Applications


Applications

Species

Dilution

Positive Tissue

WB

--

1: 500-1: 1000

Ttransfected 293T cells



Background


The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.




Images


图片 1.png

Western blot analysis of FLAG Tag (GB11938) 

at dilution of 1: 1000

Lane 1: 293T cells transfected with empty vector 

Lane 2: 293T cells transfected with vector overexpressing Flag-tag fusion protein 




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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