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产品详情
操作说明
注意事项
Product Information
|
Description |
EGFP rabbit polyclonal |
|
Protein full name |
Enhanced GFP |
|
Synonyms |
Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP |
|
Immunogen |
Recombinant protein corresponding to EGFP( enhanced green fluorescent protein) |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. |
27 kDa |
|
Observed MW. |
29 kDa |
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
/ |
1: 500-1: 1000 |
cells/ tissues with eGFP |
|
IF |
/ |
1: 500-1: 1000 |
293T |
Background
Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining. Other applications of eGFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
Images
|
Western blot analysis of eGFP (GB11602) at dilution of 1: 500 Lane 1: 293T cell lysate Lane 2: 293T with eGFP cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 with eGFP cell lysate |
|
Immunofluorescence analysis of human 293T cells using eGFP (GB11602) at dilution of 1: 1000 A: DAPI nuclear stain ( blue ) B: eGFP protein ( green ) C: Anti-eGFP ( red ) D: Merged |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
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地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室