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Anti -Histone H2A.X Rabbit pAb
价格：
在线咨询
货号：
KT012183
产品规格：
Applications：
WB/IHC/IF
Species：
H/M/R,H/M/R,H/M/R
购买数量：
产品详情
操作说明
注意事项
Product Information
|
Description |
Histone H2A.X rabbit polyclonal |
|
Protein full name |
Histone H2AX |
|
Synonyms |
H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Human Histone H2A.X |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. |
15 kDa |
|
Observed MW. |
15 kDa |
|
Subcellular location |
Nucleus |
|
Uniprot ID |
P16104, P27661 |
Applications
|
applications |
species |
dilution |
Positive tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
liver, kidney, colon |
|
IHC |
Human, Mouse, Rat |
1: 1000-1: 2000 |
colon cancer, colon, kidney, testis |
|
IF |
Human, Mouse, Rat |
1: 500-1: 1000 |
colon cancer, colon, kidney, testis |
Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Images
|
Western blot analysis of H2AX (GB111508) at dilution of 1: 1000 Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Mouse colon tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Rat kidney tissue lysate |
|
Immunohistochemistry analysis of paraffin-embedded human colon cancer using H2AX (GB111508) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse colon using H2AX (GB111508) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded rat colon using H2AX (GB111508) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse kidney using H2AX (GB111508) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded rat kidney using H2AX (GB111508) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse testis using H2AX (GB111508) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded rat testis using H2AX (GB111508) at dilution of 1: 2000 |
|
Immunofluorescent analysis of paraformaldehyde-fixed human colon cancer using H2AX (GB111508) at dilution of 1: 600 |
|
Immunofluorescent analysis of paraformaldehyde-fixed rat colon using H2AX (GB111508) at dilution of 1: 600 |
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse kidney using H2AX (GB111508) at dilution of 1: 600 |
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using H2AX (GB111508) at dilution of 1: 600 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室