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产品详情
操作说明
注意事项
Product Information
|
Description |
alpha Tubulin mouse monoclonal |
|
Protein full name |
Tubulin alpha-1A chain |
|
Synonyms |
TUBA1A, B-ALPHA-1, LIS3, TUBA3, tubulin alpha 1a,TUBA1B, K-ALPHA-1, tubulin alpha 1b |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse alpha Tubulin |
|
Isotype |
IgG2b |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoskeleton |
|
Predicted MW. |
50 kDa |
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Observed MW. |
55 kDa |
|
Uniprot ID |
Applications
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Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 2000-1: 4000 |
lung, brain |
|
IHC |
Human, Mouse, Rat |
1: 1000-1: 2000 |
|
|
IF |
Human, Mouse, Rat |
1: 1000-1: 2000 |
kidney, fibrogenic tumor, brain, lung, kidney |
Background
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly.
Images
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Western blot analysis of alpha Tubulin (GB12200) at dilution of 1: 4000 Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: NIH3T3 cell lysate Lane 5: C6 cell lysate Lane 6: Mouse lung tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat lung tissue lysate Lane 9: Rat brain tissue lysate |
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Immunofluorescent analysis of paraformaldehyde-fixed human kidney using alpha Tubulin (GB12200) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed human fibrogenic tumor using alpha Tubulin (GB12200) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using alpha Tubulin (GB12200) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse lung using alpha Tubulin (GB12200) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat lung using alpha Tubulin (GB12200) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse kidney using alpha Tubulin (GB12200) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat kidney using alpha Tubulin (GB12200) at dilution of 1: 2000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室