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Product Information
Description |
SARS-CoV-2 spike glycoprotein mouse monoclonal |
Protein full name |
SARS-COV-2 S protein S1 subunit RBD domain |
Synonyms |
SARS-COV-2 S protein S1, RBD, SARS-CoV-2 spike glycoprotein, ARS-CoV-2 S protein RBD, Covid-19 |
Immunogen |
SARS-COV-2 S protein S1 subunit RBD domain recombinant protein expressed in 293F cells. |
Isotype |
IgG |
Purity |
Affinity purification |
Predicted MW. |
25 kDa |
Observed MW. |
25 kDa/35 kDa (glycosylation) |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
----------- |
1: 500-1: 1000 |
Use at an assay dependent sample |
Elisa |
----------- |
1: 500-1: 20000 |
----------- |
Background
SARS-COV-2 S protein S1 is a powerful weapon of coronavirus SARS-COV-2 to infect human normal cell. Because the receptor binding domain (RBD) lies in S spike protein S1 subunit, which exclusively binds to human ACES2 protein, followed by SARS-COV-2 nuclear materials entering into human cells cytoplasm and starting replication.
Images
|
Western blot analysis of SARS-COV-2 S protein S1 (GB12869) at dilution of 1:600. Lane 1-3: recombination RBD protein expressed in 293F cells |
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Binding Activity of GB12869 with SARS-COV-2 S protein S1. Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-COV-2 S protein S1 at 1 μg/ml can bind GB12869. The EC50 of GB12869 is 7.67 ng/ml. |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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