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产品详情
操作说明
注意事项
Product Information
| Description |
TNF Alpha mouse monoclonal |
| Protein full name |
Tumor necrosis factor alpha |
|
Synonyms |
TNF, DIF, TNF-alpha, TNFA, TNFSF2, Tumour necrosis factor, Tumour necrosis factor alpha, TNF-α, tumor necrosis factor, TNLG1 |
| Immunogen |
Recombinant protein corresponding to Human TNF α |
| Isotype | IgG |
| Purity | Affinity purification |
|
Predicted MW. |
26 kDa |
|
Observed MW. |
26 kDa |
| Subcellular location |
Cell membrane,Secreted |
| Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Mouse |
1: 2000-1: 5000 |
LPS treated RAW 264.7 cells, HeLa cells,HL-60 cells, PC-12 cells |
|
IHC |
Human |
1: 200-1: 1000 |
breast cancer, colon cancer, liver cancer |
|
IF |
Human |
1: 50-1: 500 |
breast cancer |
Background
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
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Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥万物生物科技有限公司
客服热线：400-1016-218
QQ：355185756
地址：安徽省合肥市高新区黄山路602号合肥国家大学科技园A401室