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中文名称:ENPP6抗原(重组蛋白)
英文名称: ENPP6 Antigen (Recombinant Protein)
储 存: 冷冻(-20℃)
相关类别: 抗原
概述
Fusion protein corresponding to a region derived from 119-419 amino acids of human ENPP6 |
技术规格
Full name: |
ectonucleotide pyrophosphatase/phosphodiesterase 6 |
Synonyms: |
NPP6 |
Swissprot: |
Q6UWR7 |
Gene Accession: |
BC035035 |
Purity: |
>85%, as determined by Coomassie blue stained SDS-PAGE |
Expression system: |
Escherichia coli |
Tags: |
His tag C-Terminus, GST tag N-Terminus |
Background: |
Choline-specific glycerophosphodiester phosphodiesterase. Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid. |
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